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Enterobacter sakazakii: infectivity and enterotoxin production in vitro and in vivo [Article de Revue]

 
[Enterobacter sakazakii : infectivité et production d'enterotoxine in vitro et in vivo]
PAGOTTO FJ ; NAZAROWEC-WHITE M ; BIDAWID S ; FARBER JM
Journal of food protection. 2003; 66(3) : 370-375
Summary: Enterobacter sakazakii est un micro-organisme considéré comme responsable de formes sévères de méningite néonatale. Les préparations lactées en poudre ont été identifiées comme source potentielle de ce micro-organisme dans les épidémies et les cas sporadiques. Dans cette étude on a évalué la production d'entérotoxine des isolats cliniques et alimentaires d'E. sakazakii par un essai sur des souris et des essais in vitro. Ceci est le premier rapport décrivant des facteurs de virulence supposés d'E. sakazakii.

Enterobacter sakazakii has been implicated as the causal organism in a severe form of neonatal meningitis, with reported mortality rates of 40 to 80%. Dried infant formula has been identified as a potential source of the organism in both outbreaks and sporadic cases. In this study, clinical and foodborne isolates of E. sakazakii were evaluated for enterotoxin production by the suckling mouse assay. In addition, suckling mice were challenged both orally and by intraperitoneal injection. Of 18 E. sakazakii strains evaluated, four were found to test positive for enterotoxin production. All strains of E. sakazakii were lethal to suckling mice at 10(8) CFU per mouse by intraperitoneal injection, while two strains caused death by the peroral route. In in vitro assays, CHO, Vero, and Y-1 cells demonstrated both cell lysis and rounding when exposed to E. sakazakii strain LA filtrates. This is the first report describing any putative virulence factors of E. sakazakii.(RESUME D'AUTEUR)
Publication
2003

Pages
370-375

Language
Anglais

Number of ref
25

Published in
Journal of food protection

ID notice
319341

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