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The molecular epidemiology and evolution of the Panton-Valentine leukocidin-positive, methicillin-resistant Staphylococcus aureus strain USA300 in Western Australia [Article de Revue]

[Epidémiologie moléculaire et évolution de la souche USA 300 de Staphylococcus aureus résistant à la méticilline et positif pour la leucocidine de Panton-Valentine dans l'ouest australien]
Monecke, S ; Ehrich, Ralf ; SLICKERS P ; TAN HL ; COOMBS G
Clinical microbiology and infection. 2009/08; 15(8) : 770-776
Summary: Between 2003 and 2008, 76 clinical isolates of the Panton-Valentine leukocidin-positive Staphylococcus aureus strain .West Australianmethicillin-resistant Staphylococcus aureus (MRSA)-12. (WA MRSA-12) were recovered from 72 patients living in the Perth area in Western Australia. These isolates were found to belong to multilocus sequence type 8, and had a USA300-like pulsed-field gel electrophoresis pulsotype. All isolates were genotyped using diagnostic DNA arrays covering species markers, resistance factors, virulenceassociated, as well as MSCRAMM (microbial surface components recognizing adhesive matrix molecules) genes to prove the identitybetween WA MRSA-12 and the pandemic strain USA300, as well as to detect possible genetic variability. In general, WA MRSA-12 isolates were similar to USA300, and the most common variant was identical to USA300-TC1516. From this clone, most of the other variants may have evolved by a limited number of gene losses or acquisitions. Variations in carriage of virulence and resistance- associated genes allow distinction of variants or sub-clones. Altogether, 16 variants could be distinguished. They differed in the carriage of resistance genes (blaZ/I/R, ermC, msrA + mpbBM, aadD + mupR, aphA3 + sat, tetK, qacC, merA/B/R/T) of beta-haemolysin-converting phages and of enterotoxins (sek + seq, which were deleted in four isolates). Notably, the arginine catabolic mobile element (ACME) was absent in 12 isolates (15.8%). The mercury resistance (mer) operon, which is usually associated with SCCmec type III elements, was found inseveral ACME-negative isolates. The present study emphasises the importance of genotyping in detecting the introduction and evolution of significant MRSA strains within a community. (RESUME D'AUTEUR)



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Clinical microbiology and infection

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